UV-1300 UV-visible spectrophotometric determination

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UV-1300 UV-Vis Spectrophotometric Determination

Keywords: UV-1300 UV-visible spectrophotometer phenol analyzer

$Width="1023" height="766" src="http://i.bosscdn.com/blog/nophoto.gif" /></p> First, the purpose of the experiment 1, understand the structure of the UV-visible spectrophotometer, Performance and method of use 2. Familiar with qualitative and quantitative methods 2. Experimental principle Ultraviolet Spectrophtometry, also known as Ultraviolet Moleculor Absorption Spectrophtometry, is to study the molecular absorption range of 190.0~1100.0nm. The absorption spectrum inside. Ultraviolet absorption spectroscopy is mainly caused by the transition of molecular valence electrons between electron energy levels, and is an analytical method for studying the electronic spectrum of matter. By measuring the absorption of ultraviolet light by molecules, a large number of inorganic and organic substances can be qualitatively and quantitatively determined. Phenol is a highly toxic substance that can cause cancer and has been included in the blacklist of organic pollutants. However, some drugs, food additives, disinfectants and other products contain a certain amount of phenol. If the content exceeds the standard, it will have a great toxic effect. The maximum absorption wavelength of phenol in the ultraviolet region is Î»max = 270 nm. When the phenol solution was scanned, there was a strong absorption peak at 270 nm. For qualitative analysis, the standard sample and the unknown sample can be scanned for wavelength under the same conditions, and the unknown sample can be identified by comparing the spectra of the unknown sample and the standard sample. In the absence of standard samples, comparisons can be made according to standard spectra or related electronic spectral data sheets. The quantitative analysis is the absorbance of a standard sample of different concentrations of phenol measured at 270 nm, and a standard curve is automatically drawn. The absorbance value of the unknown sample was measured under the same conditions, and the phenol content in the unknown sample was obtained from the standard curve. Third, instruments and reagents 1. UV-1300 UV-visible spectrophotometer (American company) 2. Volumetric flask (1000ml, 250ml) 3. Colorimetric tube (50ml) 4. Pipette (5ml, 10ml) 5. Phenol (AR) accurately weighed 1.000 g of phenol in 200 ml of distilled water, dissolved and quantitatively transferred to a 1000 ml volumetric flask as a stock solution. Fourth, the experimental steps 1. Turn on the instrument and computer, monitor, printer power, enter the "UV Professional" software operating system, wait for the instrument to self-test, click OK, enter the main operation page. 2. Wavelength scanning (1) Determination of wavelength scanning parameters: slit width 1.5nm photometric form absorption value scanning range 200 ~ 500nm scanning speed 1000nm / min data interval point 1nm storage file selection path and file name (such as F: \ phenol.UVD) (2) Make a baseline. Place the cuvette containing the reference solution into the reference light path and the sample light path. Click â€œBaselineâ€ to start the baseline scan. (3) Wavelength scan. The cuvette containing the sample and the reference solution. Place the reference light path and the sample light path separately, click â€œScanâ€ to start scanning. (4) Qualitative analysis compare the wavelength scan of the sample with the known wavelength scan or the known spectrum under the same conditions. Qualitative analysis of the sample 3. Quantitative analysis (1) The standard series was prepared in 5 50ml colorimetric tubes. Add 0.5 ml, 2 ml, 5 ml, 10 ml, 20 ml of 10 ug/ml phenol standard solution with a pipette, and dilute to the mark with distilled water. Shake well. (2) Determine the quantitative analysis parameter setting a: Method Repeat reading times for each standard 1 Repeat reading times for each sample 1 Is it necessary to measure the standard sample Need calibration curve Linear calculation method Calculate the quantitative value by peak height b: The total number of samples to be sampled. 6 Enter the name of the sample to be tested. The phenol sample label is edited from the first number. 1 Input standard sample concentration value. Standard sample is identified in the std column. c: Quality control requires quality control. After the measurement result is marked, continue to measure the maximum allowable concentration input. The minimum allowable concentration d: Instrument slit 1.5nm measurement form absorbance intensity multiple 1 working wavelength 270nm integration time 5s to inject blank liquid into two cuvettes, and respectively Put the reference and sample light path, press â€œzeroâ€ to zero, then press â€œOKâ€ e: report needs report online report f: result storage input selection path and file name (3) Press â€œRUNâ€ to start quantitative measurement Follow the prompts to place each sample (4) Press â€œViewâ€ to observe the standard, sample and calibration curve. 5. Discussion of Questions 1. Purple 2. What are the main components of the UV-Vis spectrophotometer? 3. Explain the characteristics and application range of UV-visible spectrophotometry. 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