Shanghai ancient flower detailed description: ELISA kit OD value is not normal - Database & Sql Blog Articles

XSA00732AFG1H-OD

Test - lowercase jpg

Kaixin micro test

Test probe P100-M3

F380J106MMA original authentic, spot stock brand NICHICON

Due to the complicated operation steps of ELISA and the influence of many reaction factors, some large and small problems have appeared in the experimental problems. However, these problems can also be avoided, and it will be much simpler after mastering. Recently, a customer asked the ELISA question: What is the abnormal OD value? What are the reasons? Shanghai Guduo Biotechnology is

The OD value is not normal to find the reason, I hope to help you!

1. The kit has not been warmed up (corresponding solution: the kit is warmed to 25 ° C);

2. The temperature control is not good (corresponding solution: control the correct temperature);

3. Incubation time is not accurate (corresponding solution: control incubation time);

4. The impact force is too large when washing, the soaking time is too long, and the number of washings is increased (corresponding solution: washing 4-5 times, 250ul per hole, then patted dry);

5. Direct sunlight, blowing directly against the wind (corresponding to the solution: avoiding the wind and avoiding the sun);

6. The wavelength of the microplate reader is wrong. Elisa kit (corresponding solution: the wavelength of the microplate reader is 450 nm);

7. Antibody dilution error (corresponding solution: correct dilution of antibody);

8. Water quality problem (corresponding to the solution: replace it with Wahaha mineral water).

Finally, the company wants to say here: "No matter what experiment, there are excellent options for it. This includes the use of funds, including the loss of manpower time. If necessary, you can predict the output of your experiment and The ratio of the input, the bigger the ratio, the better, of course, based on the experimental conditions you have."