Horse coup: how to identify the advantages and disadvantages of elisa kit - Database & Sql Blog Articles

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Jinma coup:

How to identify the pros and cons of elisa kit

For the experiment of the elisa method, friends know that it is highly sensitive and reproducible, and because of this, it has been favored by researchers in scientific research. And now the suppliers are numerous and the species are very complete.

It is because of

The popularity of the elisa kit has led to many counterfeit and shoddy products on the market.

This makes many friends

The headache is endless. If the luck is not good that day, the reagents purchased are fakes, and the precious samples collected by the hard work are destroyed.

,

How to distinguish quality

Elisa kit,

Can't pick which kit is best for you.

This of course must be handed over to the sales champion: Shanghai Jinma Experimental Equipment Co., Ltd. advises everyone:

method one:

Selecting a concentration within the detection range to make a porous repeat shows that the parallelism is good. That is, the size of the CV value in the board, the accuracy of the sample loading is required for this operation. For some rougher kits, the inter-plate CV values ​​are larger.

Method Two:

A known concentration of recombinant cytokine can be used and diluted into the detection range as a sample to see the accuracy of the test.

Method three:

The sensitivity can be measured by diluting the minimum concentration calibrated on the kit using the recombinant cytokine to be tested. It is recommended to have more than 5 replicates. The average manufacturer uses the results of 20 duplicate wells as the detection limit. Using this experiment, it can be verified whether the sensitivity of the purchased kit is as described in the specification, and whether the purchased kit is exaggerated or not.

Method four:

If the kit has the same index, the other standard can be used as a sample, and each kit is added for testing to detect the authenticity and accuracy of the kit. If one of them is well-known and reliable, it can be used as a standard to verify the accuracy of another kit.

Jinma prepares for you

Five points, I also hope to help friends when they buy.

1. It is clear that the gene encoding the protein has the best homology with other species.

2. Find kits for detecting proteins from these species.

3. Identify which proteins are detected.

4. Make your own judgment which one to buy.

5, consult the supplier elisa is the type of antibody used in the kit: polyclonal or monoclonal antibody, the monoclonal antibody is determined by what antigen.

Shanghai Jinma Experimental Equipment Co., Ltd. provides you with high quality

Elisa kit products, I wish you success!